Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study

Aim: In vitro evaluation of the influence bovine xenogenic biomaterials on stem cells from human exfoliated deciduous teeth (SHEDs). The study was divided into three groups: 1) group C (control), containing only MSCs; 2) BP, MSCs and Bonefill Porous®; 3) BO, Bio-Oss®. were derived a tooth 7-year-old male donor. An aliquot subjected to immunophenotyping by flow cytometry. Cell viability (neutral red), cytotoxicity (MTT), cell proliferation (crystal violet) assays performed. All groups underwent morphological analysis light microscopy (LM), biomaterial with superior performance submitted scanning electron (SEM). Time points 24, 48, 72 h culture used. results evaluated significance level 0.05. Results showed that both maintained similar control. BO smaller compared other groups. LM evaluation, BP more spread adherent than group. SEM, characteristics active those Bovine positively influenced SHEDs, while seemed present higher potential SHEDs for future application within in vivo and/or clinical studies.